Multiple proteins encoded within the urease gene complex of Proteus mirabilis
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چکیده
منابع مشابه
H-NS is a repressor of the Proteus mirabilis urease transcriptional activator gene ureR.
Expression of Proteus mirabilis urease is governed by UreR, an AraC-like positive transcriptional activator. A poly(A) tract nucleotide sequence, consisting of A(6)TA(2)CA(2)TGGTA(5)GA(6)TGA(5), is located 16 bp upstream of the sigma(70)-like ureR promoter P2. Since poly(A) tracts of DNA serve as binding sites for the gene repressor histone-like nucleoid structuring protein (H-NS), we measured ...
متن کاملInteraction of Proteus mirabilis urease apoenzyme and accessory proteins identified with yeast two-hybrid technology.
Proteus mirabilis, a gram-negative bacterium associated with complicated urinary tract infections, produces a metalloenzyme urease which hydrolyzes urea to ammonia and carbon dioxide. The apourease is comprised of three structural subunits, UreA, UreB, and UreC, assembled as a homotrimer of individual UreABC heterotrimers (UreABC)(3). To become catalytically active, apourease acquires divalent ...
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Chromosome-Encoded Class D -Lactamase OXA-23 in Proteus mirabilis R. Bonnet,* H. Marchandin, C. Chanal, D. Sirot, R. Labia, C. De Champs, E. Jumas-Bilak, and J. Sirot Laboratoire de Bactériologie, Faculté de Médecine, 63001 Clermont-Ferrand Cedex, Laboratoire de Bactériologie, Hôpital Arnaud de Villeneuve, 34295 Montpellier Cedex 5, UMR 175, CNRS-MNHN, 29000 Quimper, Laboratoire de Bactériologi...
متن کاملVisualization of Proteus mirabilis within the matrix of urease-induced bladder stones during experimental urinary tract infection.
The virulence of a urease-negative mutant of uropathogenic Proteus mirabilis and its wild-type parent strain was assessed by using a CBA mouse model of catheterized urinary tract infection. Overall, catheterized mice were significantly more susceptible than uncatheterized mice to infection by wild-type P. mirabilis. At a high inoculum, the urease-negative mutant successfully colonized bladders ...
متن کاملProteus mirabilis urease: histidine 320 of UreC is essential for urea hydrolysis and nickel ion binding within the native enzyme.
Proteus mirabilis urease, a nickel-containing enzyme, has been established as a critical virulence determinant in urinary tract infection. An amino acid sequence (residues 308 to 327: TVDEHLDMLMVCHHLDPSIP) within the large urease subunit, UreC, is highly conserved for every urease examined thus far and has been suggested to reside within the enzyme active site. Histidine residues have been post...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1988
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.170.3.1027-1033.1988